{"id":2621,"date":"2020-04-09T05:45:07","date_gmt":"2020-04-08T21:45:07","guid":{"rendered":"https:\/\/fexstudio.com\/?p=2621"},"modified":"2023-05-27T08:03:57","modified_gmt":"2023-05-27T00:03:57","slug":"%e6%96%b0%e5%86%a0%e8%82%ba%e7%82%8e%e7%9a%84%e5%9f%ba%e5%9b%a0%e8%a8%ba%e6%96%b7%e6%80%8e%e9%ba%bc%e6%aa%a2%e6%b8%ac%e7%9a%84%ef%bc%9f","status":"publish","type":"post","link":"https:\/\/fexstudio.com\/?p=2621","title":{"rendered":"\u65b0\u51a0\u80ba\u708e\u7684\u57fa\u56e0\u8a3a\u65b7\u600e\u9ebc\u6aa2\u6e2c\u7684\uff1f"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"2621\" class=\"elementor elementor-2621\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e888571 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"e888571\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-4ae658f\" data-id=\"4ae658f\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-80878e4 elementor-widget elementor-widget-heading\" data-id=\"80878e4\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u88ab\u61f7\u7591\u611f\u67d3\u65b0\u51a0\u75c5\u6bd2\uff0c\u7136\u5f8c\u53d6\u6aa2\u9ad4\uff0c\u7136\u5f8c\u57fa\u56e0\u6aa2\u6e2c\u662f\u5565\uff1f<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a721214 elementor-widget elementor-widget-text-editor\" data-id=\"a721214\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3><span style=\"text-decoration: underline;\"><span style=\"color: #0000ff; text-decoration: underline;\"><strong><a style=\"color: #0000ff; text-decoration: underline;\" href=\"https:\/\/www.facebook.com\/pg\/fexstudio555\/posts\/\">\u2286\u2660\u8ffd\u8e2a\u7814\u6b21\u65b9FB\u7c89\u7d72\u5c08\u9801\u770b\u66f4\u591a\u6709\u8da3\u7684\u4e8b\u7269!\u2660\u2287<\/a><\/strong><\/span><\/span><\/h3>\n<p>\u73fe\u968e\u6bb5\u5927\u90e8\u5206\u7684 \u65b0\u51a0\u75c5\u6bd2\u548c \u6d41\u611f\u7684\u8a3a\u65b7\u662f\u7528 <span style=\"text-decoration: underline; color: #ff0000;\"><strong>\u57fa\u56e0\u7be9\u6aa2<\/strong><\/span> \u7684\uff0c\u90a3\u9019\u500b\u7be9\u6aa2\u600e\u9ebc\u9032\u884c\u7684\u5462\uff1f\u5c31\u662f\u53d6\u5f97\u53d7\u6aa2\u8005\u7684\u6aa2\u9ad4\uff0c\u4f46\u662f\u56e0\u70ba\u63a1\u6a23\u7684\u6642\u5019\u5c31\u53ea\u662f\u62ff\u7279\u6b8a\u63a1\u6a23\u201c\u68c9\u82b1\u68d2\u201d\uff0c\u6240\u4ee5\u75c5\u6bd2\u7684\u57fa\u56e0\u91cf\u901a\u5e38\u90fd\u6703\u975e\u5e38\u7684\u5fae\u91cf\uff0c\u5c0e\u81f4\u6aa2\u6e2c\u96e3\u5ea6\u975e\u5e38\u9ad8\uff0c\u90a3\u600e\u9ebc\u8fa6\u5462\uff1f\u6709\u4e00\u500b\u65b9\u6cd5\u7c21\u55ae\u4e14\u4fbf\u5b9c\uff0c\u90a3\u5c31\u662f\u628a\u5b83\u8907\u88fd\u5f88\u591a\u6b21\u4e0d\u5c31\u597d\u4e86\uff0c\u6c92\u932f\uff5e\u4f46\u600e\u9ebc\u9032\u884c\u5462\uff1f\u554a\u54c8\uff01\uff01\uff01\uff01\u4f7f\u7528\u4e00\u500b\u6280\u8853\u53eb\u505aPCR\uff08\u5168\u540d\uff1aPolymerase Chain Reaction\uff0c\u7ffb\u8b6f\uff1a\u805a\u5408\u9176\u93c8\u9396\u53cd\u61c9\uff09\uff0c\u6211\u9084\u662f\u7528PCR\u4f86\u4ee3\u8868\u5427\uff0c\u7c21\u55ae\u4f86\u8aaaPCR\uff0c\u5c31\u662f \u201c\u57fa\u56e0\u7684\u8907\u5370\u6a5f\u201d \uff01\uff01<\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/i.makeagif.com\/media\/4-27-2015\/7b9lVN.gif\" alt=\"Nasal Swab on Make a GIF\" width=\"612\" height=\"345\"><\/p>\n<figure style=\"width: 613px\" class=\"wp-caption aligncenter\"><img decoding=\"async\" src=\"https:\/\/www.thebeijinger.com\/sites\/default\/files\/naso1_0.gif\" alt=\"Mandatory Nucleic Acid Tests for International Arrivals: What to ...\" width=\"613\" height=\"346\"><figcaption class=\"wp-caption-text\">\u9f3b\u8154\u7684\u6aa2\u9ad4\u63a1\u6a23\uff0c\u8981\u5fcd\u4f4f\u4e0d\u6253\u5674\u568f\uff01\uff01\u770b\u8d77\u4f86\u5c31\u5f88\u4e0d\u8212\u670d\uff5e\u4e8b\u5be6\u4e0a\u5c31\u662f\u62ff\u5169\u500d\u9577\u7684 <span style=\"color: #ff0000;\"><strong>\u68c9\u82b1\u68d2 <\/strong><\/span>\u5f80\u9f3b\u5b50\u88e1\u9762\u9001\uff5e<\/figcaption><\/figure>\n<p>PCR\u7684\u904b\u7528\u975e\u5e38\u5ee3\uff0c\u53ea\u8981\u662f\u9700\u8981\u5c07\u975e\u5e38\u5fae\u91cf\u7684\u6aa2\u9ad4\uff0c\u8907\u88fd\u500d\u589e\u5de8\u5927\u5316\u7684\u904b\u7528\u90fd\u548c\u4ed6\u6709\u95dc\uff0c\u4e0d\u662f\u53ea\u6709\u8a3a\u65b7 \u65b0\u51a0\u75c5\u6bd2 \u548c \u6d41\u611f \u800c\u5df2\u54e6\uff01\u5176\u4ed6\u5305\u62ec\u4e86\uff0c\u72af\u7f6a\u9451\u8b58\u79d1\u5b78\uff0c\u57fa\u56e0\u6392\u5e8f\uff0c\u5fae\u751f\u7269\u7814\u7a76\uff0c\u5176\u5b83\u75be\u75c5\u8a3a\u65b7\uff08\u6bd4\u5982\u8aaa \u611b\u6ecb\u75c5\uff09\uff0c\u751a\u81f3\u9084\u6709 <span style=\"color: #000000;\">\u8003\u53e4<\/span>\uff08<span style=\"color: #ff0000;\"><strong>\u7814\u7a76\u5316\u77f3\u7684DNA<\/strong><\/span>\uff09<\/p>\n<p>\u9019\u500b\u5c31\u662f\u5176\u4e2d\u4e00\u7a2ePCR\u6a5f\u5668\uff0c\u578b\u865f\uff1aSimpliAmp Thermal Cycler\uff0c\u4e2d\u6587\u76f4\u63a5\u7ffb\u8b6f\u53eb\u505a\u5faa\u74b0\u52a0\u71b1\u5668\uff0c\u70ba\u4ec0\u9ebc\u53eb\u5faa\u74b0\u52a0\u71b1\u5668\u5462\uff1f\u4e8b\u5be6\u4e0a\u4ed6\u771f\u7684\u5c31\u5f88\u50cf<span style=\"color: #ff0000;\"><strong>\u8212\u80a5\u6a5f<\/strong><\/span>\uff0c\u63a5\u4e0b\u6211\u4f86\u6703\u89e3\u91cb\uff0c\u8b93\u6211\u5011\u7e7c\u7e8c\u770b\u4e0b\u53bb\uff5e<\/p>\n<p><img decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.kimforest.com\/upload\/product\/pic\/20160823174432wee.jpg\" width=\"651\" height=\"342\"><\/p>\n<p>PCR\u7684\u4f5c\u7528\u539f\u7406\u4e8b\u5be6\u4e0a\u4e26\u4e0d\u96e3\u7406\u89e3\uff0c\u5c31\u662f\u628a\u975e\u5e38\u5c11\u91cf\u7684\u57fa\u56e0\uff0c\u201c\u5f71\u5370\u8907\u88fd\u201d\uff0c\u9054\u5230\u8db3\u4ee5\u5075\u67e5\u5230\u7684\u91cf\uff0c\u6240\u4ee5\u57fa\u672c\u4e0a\uff0c\u5728PCR\u6a5f\u5668\u88e1\u9762\u767c\u751f\u7684\u4e8b\u60c5\uff0c\u5c31\u662f\u4e00\u9023\u4e32\u5c07\u975e\u5e38\u5c11\u91cf\u7684\u57fa\u56e0 \u201c\u8907\u88fd\u201d \u7684\u904e\u7a0b\uff0c\u4e00\u5206\u70ba\u4e8c\uff0c\u4e8c\u5206\u70ba\u56db\uff0c\u56db\u5206\u70ba\u516b\uff0c\u5728\u9019\u500b\u904e\u7a0b\u4e2d\uff0c\u63a7\u6eab\u662f\u4e00\u500b\u95dc\u9375\uff0c\u6240\u4ee5<span style=\"text-decoration: underline; color: #ff0000;\"><strong>\u5c31\u50cf\u8212\u80a5\u6a5f<\/strong><\/span>\u4e00\u6a23\uff0c\u5728\u56fa\u5b9a\u7684\u6642\u9593 \u63d0\u4f9b \u56fa\u5b9a\u7684\u6eab\u5ea6\uff5e \u5c07\u4e00\u6bb5\u57fa\u56e0\u8907\u88fd20\u523040\u6b21\uff0c\u807d\u8d77\u4f86\u4e0d\u591a\uff1f \u7b97\u7d66\u4f60\u770b\uff0c\u4e00\u6bb5\u57fa\u56e0\uff0c\u5982\u679c\u88ab\u8907\u88fd20\u6b21\uff0c\u5b83\u5c07\u6703\u8b8a\u6210 2\u768420\u6b21\u65b9\uff0c\u90a3\u5c31\u662f105\u842c\u500d\uff0c\u90a3\u5982\u679c\u8907\u88fd40\u6b21\uff0c\u4ed6\u5c31\u6703\u8b8a\u62102\u768440\u6b21\u65b9\uff0c\u90a3\u5c31\u662f <span style=\"text-decoration: underline;\"><span style=\"color: #ff00ff; text-decoration: underline;\"><strong>1\u5146\u500d\u4ee5\u4e0a<\/strong><\/span><\/span>\uff0c\u9019\u6a23\u7684\u8907\u88fd\u91cf\u5c31\u6703\u5c07\u8a3a\u65b7\u8b8a\u5f97\u76f8\u5c0d\u5bb9\u6613\uff01\u4f46\u662f\u7576\u7136\u4e5f\u4e0d\u662f\u8d8a\u591a\u6b21\u8d8a\u597d\uff0c\u56e0\u70ba\uff0c\u53ea\u8981\u662f\u8b1b\u5230\u57fa\u56e0\u7684\u8907\u88fd\uff0c\u5c31\u4e00\u5b9a\u6703\u6709 \u201c\u7a81\u8b8a\u201d \u7684\u53ef\u80fd\uff0c\u7121\u8ad6\u662f\u5728\u771f\u7684\u7d30\u80de\u88e1\u9762\u9084\u662f\u5728PCR\u6a5f\u5668\u88e1\u9762\uff0c\u8907\u88fd\u7d04\u591a\u6b21\uff0c\u932f\u8aa4\u7684\u6a5f\u6703\u5c31\u8d8a\u9ad8\uff0c\u6240\u4ee5\u6bcf\u4e00\u7a2e\u985e\u578b\u7684\u6aa2\u9ad4\u7684\u8907\u5236\u90fd\u6709\u6700\u9069\u7576\u7684SOP\uff0c\u90a3\u81f3\u65bc\u8907\u88fd\u7684\u57fa\u672c\u6982\u5ff5\u5462\uff1f\u6211\u5011\u4e0b\u9762\u5c31\u6703\u4e00\u6b65\u4e00\u6b65\u7528\u5716\u6848\u548c\u52d5\u756b\u89e3\u91cb\u54e6\uff5e\uff08<strong>\u9019\u88e1\u6211\u7528<span style=\"text-decoration: underline;\">\u96d9\u80a1DNA<\/span>\u505a\u4f8b\u5b50\uff0c\u56e0\u70ba\u9019\u6a23\u89e3\u91cb\u6703\u6bd4\u8f03detail<\/strong>\uff09<\/p>\n<p>\u57fa\u672c\u4e0a PCR \u9808\u5177\u5099\u516d\u500b\u8981\u7d20\uff1a<\/p>\n<p>1. \u6b32\u8907\u88fd\u7684 DNA \u6aa2\u9ad4\uff08<span style=\"color: #ff0000;\"><strong>Sample<\/strong><\/span>\uff09\uff0c\u9019\u5c31\u662f\u6211\u5011\u7684\u539f\u7a3f\uff0c\u9700\u8981\u8907\u88fd\u7684\u6771\u897f\uff0c\u4e5f\u5c31\u662f\u5f9e\u75c5\u4eba\u9f3b\u8154\u5167\u53d6\u51fa\u7684\u6aa2\u9ad4\uff5e<\/p>\n<p>2. \u8d77\u9ede\u8207\u7d42\u9ede\u7684\u5f15\u5b50(<span style=\"color: #ff0000;\"><strong>Primers<\/strong><\/span>)\uff1a\u56e0\u70ba\u6211\u5011\u4e0d\u662f\u8981\u8907\u88fd\u6574\u689d\u57fa\u56e0\uff0c\u6211\u5011\u53ea\u662f\u9700\u8981\u5fa9\u5236\u67d0\u4e00\u6bb5\uff0c\u90a3\u5f15\u5b50\u5c31\u662f\u76ee\u6a19\u6bb5\u7684 \u201c\u958b\u59cb\u201d \u8207 \u201c\u7d50\u5c3e\u201d \u9ede\uff5e<\/p>\n<p>3. DNA \u805a\u5408\u9176(<span style=\"color: #ff0000;\"><strong>Taq Polymearse<\/strong><\/span>)\uff0c\u9019\u500b\u5c31\u662f\u771f\u6b63\u7684 \u201c\u8907\u5370\u6a5f\u201d \u4e86\uff0c\u771f\u6b63\u5728\u8907\u88fd\u57fa\u56e0\u7684\u5c31\u662f\u9019\u500b\u805a\u5408\u9176\u4e86\uff0c\u5b83\u6703\u5c07 \u201c\u58a8\u6c34\u201d \u4e00\u6ef4\u4e00\u6ef4 \u201c\u8907\u5370\u201d \u4e0a\u53bb<\/p>\n<p>4. \u57fa\u56e0\u7684\u539f\uf9be\uff08<span style=\"color: #ff0000;\"><strong>nucleic acid<\/strong><\/span>\uff09\uff0c\u9019\u500b\u5c31\u662f\u58a8\u6c34\u4e86\uff0c\u6211\u5011\u7684DNA\u96d6\u7136\u90a3\u9ebc\u9577\uff0c\u90a3\u9ebc\u591a\uff0c\u4e8b\u5be6\u4e0a\u5c31\u53ea\u542b\u6709\u56db\u7a2e\u539f\u6599\uff0c\u5f88\u5c11\uff1f\u4f60\u96fb\u8166\u7684\u5217\u5370\u6a5f\u4e5f\u53ea\u6709\u56db\u7a2e\u984f\u8272\uff08<strong><span style=\"color: #dede1f;\">\u9ec3<\/span> <span style=\"color: #0000ff;\">\u85cd<\/span> <span style=\"color: #ff0000;\">\u7d05<\/span> \u9ed1<\/strong>\uff09\uff0c\u4f60\u7684\u624b\u6a5f\u87a2\u5e55\u53ea\u6709\u4e09\u7a2e\u984f\u8272\uff08<strong><span style=\"color: #ff0000;\">\u7d05 <\/span><span style=\"color: #0de01f;\">\u7da0 <\/span><span style=\"color: #0000ff;\">\u85cd<\/span><\/strong>\uff09\uff0c\u800c\u4f60\u7684\u96fb\u8166\u4e5f\u53ea\u61c2\u5169\u500b\u5b57\uff080\u548c1\uff09\u800c\u5df2\u554a\uff01<\/p>\n<figure style=\"width: 613px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"\" src=\"https:\/\/i.imgur.com\/tT0sReF.png\" alt=\"\u56db\u8272\u58a8\u6c34\u593e\" width=\"613\" height=\"613\"><figcaption class=\"wp-caption-text\">\u96fb\u8166\u5217\u5370\u6a5f\u5c31\u662f\u7528\u9019<span style=\"color: #ff0000;\"><strong>\u56db\u7a2e\u984f\u8272<\/strong><\/span>\uff0c\u4fbf\u80fd\u6df7\u5408\u51fa\u6240\u6709\u7684\u984f\u8272\uff01\uff01<\/figcaption><\/figure>\n<p>5. PCR\u6240\u9700\u8981\u7684\u9178\u9e7c\u7de9\u6c96\u5291\uff08<span style=\"color: #ff0000;\"><strong>Buffer Solution<\/strong><\/span>\uff09<\/p>\n<p>6. PCR\u6a5f\u5668\u7684\u5c08\u7528\u5bb9\u5668<\/p>\n<p>PCR \u7684\u6aa2\u9ad4\u548c\u5404\u7a2e\u8a66\u5291\u6e96\u5099\u968e\u6bb5\u5b8c\u6210\u5f8c\u5c07\u6703\u88ab\u88dd\u5165\u5c08\u7528\u5bb9\u5668\uff0c\u7136\u5f8c\u6e96\u5099\u9001\u5165 \u201c\u8212\u80a5\u6a5f\u201d \u958b\u59cb\u53cd\u61c9\uff1a<\/p>\n<figure style=\"width: 612px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"\" src=\"https:\/\/i.imgur.com\/IpIqrFS.jpg\" alt=\"\" width=\"612\" height=\"513\"><figcaption class=\"wp-caption-text\">PCR \u7684\u6aa2\u9ad4\u548c\u5404\u7a2e\u8a66\u5291\u6e96\u5099\u968e\u6bb5\u5b8c\u6210\u5f8c\u5c07\u6703\u88ab\u88dd\u9032\u9019\u6a23\u7684\u5c0f\u7f50\u5b50\u88e1\u9762<\/figcaption><\/figure>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/i.imgur.com\/KBosT2M.jpg\" width=\"611\" height=\"762\"><\/p>\n<h2><span style=\"text-decoration: underline; color: #ff6600;\"><strong>\u90a3\u9ebc\u81f3\u65bc\u6d41\u7a0b\u662f\u4ec0\u9ebc\u5462\uff1f \u52d5\u756b\u5716\u89e3\u21d3\u21d3\u21d3\u21d3\u21d3<\/strong><\/span><\/h2>\n<p>1. \u555f\u52d5\uff08<span style=\"color: #ff0000;\"><strong>initiation<\/strong><\/span>\uff09\uff0c\u5728\u9019\u908a\u9700\u8981\u7dad\u6301\u9ad8\u6eab\uff0894\u5ea6\uff5e96\u5ea6\uff09\u7d0410\u5206\u9418\uff0c\u9019\u500b\u6eab\u5ea6\u4e3b\u8981\u662f\u53bb\u5c07\u8907\u5370\u6a5f \u201c\u6696\u6a5f\u201d \u7684\uff0c\u4e5f\u5c31\u662f\u5c07\u7b49\u7b49\u9700\u8981\u7684<span style=\"color: #ff0000;\"><strong>\u805a\u5408\u9176\u6d3b\u5316<\/strong><\/span>\uff0c\u4e26\u4e14\u628a\u75c5\u6bd2\u7834\u58de\u6389\uff0c\u4e26\u5c07\u5b83\u5011\u7684\u57fa\u56e0 \u201c<span style=\"color: #ff0000;\"><strong>\u716e\u51fa\u4f86<\/strong><\/span>\u201d<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/media.giphy.com\/media\/mEQFo9mVaQwcu0FYvd\/giphy.gif\" alt=\"Animated GIF\" width=\"613\" height=\"345\"><\/p>\n<p>2. \u8b8a\u6027 \uff08<span style=\"color: #ff0000;\"><strong>Denaturation<\/strong><\/span>\uff09\uff0c\u9019\u500b\u6b65\u9a5f\u7684\u6eab\u5ea6\u9ad8\u4e00\u9ede\u9ede\uff0c\u5178\u578b\u7684\u74b0\u5883\u662f 97\u5ea615\u79d2\uff0c\u6216\u800595\u5ea630\u79d2\uff0c\u4e3b\u8981\u662f\u9700\u5c07DNA \u201c<span style=\"color: #ff0000;\"><strong>\u716e\u958b\u4f86<\/strong><\/span>\u201d\uff5e\u6eab\u5ea6\u8981\u5920\u9ad8\u624d\u80fd\u628a\u57fa\u56e0\u716e\u958b\u4f86\uff0c\u4f46\u4e5f\u4e0d\u80fd\u716e\u592a\u4e45\u6216\u592a\u9ad8\u6eab\uff0c\u6703\u716e\u58de\u6389\uff5e<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/media.giphy.com\/media\/eMrvMZjjmR7vfXugJx\/giphy.gif\" alt=\"Animated GIF\" width=\"613\" height=\"345\"><\/p>\n<p>3. \u5c07\u5f15\u5b50\u9ecf\u4e0a\u57fa\u56e0 (Annealing of primers)\uff0c\u9019\u500b\u968e\u6bb5\u5c31\u8981\u51b7\u537b\u964d\u6eab\u4e86\uff0850~65\u5ea6\uff09\uff0c\u597d\u8b93\u5f15\u5b50\uff08primer\uff09\u53ef\u4ee5 \u201c\u9ecf\u201d \u4e0a\u8981\u5fa9\u5236\u7684\u57fa\u56e0\u6bb5\uff0c<span style=\"color: #ff0000;\"><strong>\u592a\u9ad8\u6eab\u7684\u8a71\u662f\u9ecf\u4e0d\u4e0a\u53bb\u7684<\/strong><\/span>\uff1b\u5f15\u5b50\u9ecf\u4e0a\u53bb \u201c\u8d77\u9ede\u201d \u548c \u201c\u7d42\u9ede\u201d \u4ee5\u5f8c\uff0c\u9019\u6bb5\u57fa\u56e0\u5c31\u662f\u6b32\u8907\u88fd\u7684\u57fa\u56e0\u4e86\uff0c\u57fa\u56e0\u8907\u5236\u6703\u5f9e\u8d77\u9ede\u958b\u59cb\uff0c\u7136\u5f8c\u5728\u7d42\u9ede\u7d50\u675f\uff0c\u9019\u500b\u968e\u6bb5\u5f88\u5feb\uff0c\u4e0d\u523040\u79d2\uff0c\u8d77\u9ede\u5f15\u5b50 \u548c \u7d42\u9ede\u5f15\u5b50 \u5c31\u53ef\u4ee5\u9ecf\u4e0a\u57fa\u56e0\uff01<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/media.giphy.com\/media\/XEgl9tmXlCKbd5ZY7G\/giphy.gif\" alt=\"Animated GIF\" width=\"613\" height=\"345\"><\/p>\n<p>4. \u958b\u59cb\u8907\u88fd(Elongation of primers)\uff0c\u628a\u6eab\u5ea6\u518d\u6b21\u5347\u9ad8\u81f372\u5ea6\uff0c\u9019\u500b\u6eab\u5ea6\u662f\u6700\u4f73\u5de5\u4f5c\u74b0\u5883\uff0c\u9019\u6642\u5019 \u201c<span style=\"color: #ff0000;\"><strong>\u6696\u597d\u6a5f\u7684\u8907\u5370\u6a5f<\/strong><\/span>\u201d \u5c31\u6703\u9032\u5834\uff0c\u4e26\u4e14 \u201c\u7d81\u5230\u201d \u5f15\u5b50\u548c\u57fa\u56e0\u4e0a\u9762\uff0c<span style=\"color: #ff00ff;\"><strong>\u73fe\u5728\u4f60\u5c31\u77e5\u9053 \u201c\u5f15\u5b50\u201d \u591a\u91cd\u8981\u4e86\u5427\uff01<\/strong><\/span>\u6c92\u6709 \u201c\u5f15\u5b50\u201d \u7684\u8a71\uff0c\u201c\u8907\u5370\u6a5f\u201d \u5c31\u4e0d\u77e5\u9053\u8981\u5f9e\u90a3\u908a\u958b\u59cb\u8907\u88fd\uff01<em>\u5be6<strong>\u60c5\uff1a\u805a\u5408\u9176 \u8207 \u5f15\u5b50\u548c \u57fa\u56e0 \u7d50\u5408\uff01<\/strong><\/em><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/media.giphy.com\/media\/j1mmNivxEcq2aW4D1U\/giphy.gif\" alt=\"Animated GIF\" width=\"613\" height=\"345\"><\/p>\n<p>5. \u9019\u4e9b\u8907\u5370\u6a5f\u572872\u5ea6\u7684\u74b0\u5883\u4e0b\uff0c\u6703\u4e00\u9ede\u4e00\u6ef4\u7684\u5c07\u56db\u7a2e\u984f\u8272\u7684\u58a8\u6c34\u8907\u5370\u4e0a\u6bcf\u4e00\u500b\u76f8\u5c0d\u4f4d\u7f6e\uff08\u6709\u9ede\u50cf\u62c9\u62c9\u934a\uff09\uff0c\u9019\u500b\u6b65\u9a5f\u5be6\u8cea\u4e0a \u5c31\u662f \u6d3b\u5316\u7684\u805a\u5408\u9176 \u6703\u7167\u8457\u6a21\u7d44\uff0c<span style=\"color: #ff0000;\"><strong>\u5c07\u56db\u7a2e\u6838\u82f7\u9178\u4e00\u9ede\u4e00\u6ef4\u62fc\u6e4a\u6210\u65b0\u7684\u4e00\u689d\u8907\u88fd\u54c1<\/strong><\/span>\uff01\u9019\u500b\u904e\u7a0b\u5c31\u548c\u8907\u5370\u4e00\u6a21\u4e00\u6a23\uff0c\u9019\u9ebc\u4e00\u4f86\uff0c\u539f\u672c\u4e00\u689d\u57fa\u56e0\u5c31\u8b8a\u6210\u5169\u689d\u4e86\uff01\u9019\u500b\u6b65\u9a5f\u4e3b\u8981\u9084\u662f\u8981\u770b\u6b32\u8907\u88fd\u7684\u57fa\u56e0\u6709\u591a\u9577\uff0c72\u5ea6\u7684\u74b0\u5883\u4e0b\uff0c\u5e73\u5747\u901f\u5ea6\u5927\u7d04\u843d\u5728<span style=\"color: #ff0000;\"><strong>\u6bcf\u79d2\u5ef6\u9577 35\uff5e100\u500b\u55ae\u4f4d<\/strong><\/span>\uff01<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/media.giphy.com\/media\/VEUq0sNdjmJr5LpkY9\/giphy.gif\" alt=\"Animated GIF\" width=\"613\" height=\"345\"><\/p>\n<p>6. \u9019\u9ebc\u4e00\u500b\u5faa\u74b0\u5c31\u8dd1\u5b8c\u4e86\uff0c\u6b32\u8907\u88fd\u7684\u57fa\u56e0\u5c31\u9019\u6a23double\u4e86<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/media.giphy.com\/media\/gKrbgGu0Zgtk7EvMFo\/giphy.gif\" alt=\"Animated GIF\" width=\"613\" height=\"345\"><\/p>\n<p>7. \u63a5\u8457\uff0c\u4e0b\u4e00\u500b\u5faa\u74b0\u518d\u5c07\u4e0a\u500b\u5faa\u74b0\u8907\u88fd\u51fa\u4f86\u7684\u57fa\u56e0\uff0c\u518d\u6b21double\uff0c\u9019\u6a23\u5b50\u7684\u5faa\u74b0\u6703\u91cd\u890720\u523040\u6b21\u7684 \u201c<span style=\"color: #ff0000;\"><strong>\u9023\u9396\u53cd\u61c9<\/strong><\/span>\u201d\uff0c \u628a\u5fae\u91cf\u7684\u6aa2\u9ad4\u760b\u72c2\u7684\u653e\u5927\u4e0a\u767e\u842c\u500d\uff0c\u751a\u81f3<span style=\"color: #ff0000;\"><strong>\u4e0a\u5146\u500d<\/strong><\/span>\uff0c\u9019\u500b\u9a5a\u4eba\u7684\u6578\u91cf\u5c07\u8db3\u4ee5\u8b93\u9451\u8b58\u4eba\u54e1\u505a\u8a3a\u65b7\u6216\u8005\u57fa\u56e0\u78ba\u8a8d\uff5e<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/media.giphy.com\/media\/gKlMzMC6ApIjakAzAv\/giphy.gif\" alt=\"Animated GIF\" width=\"613\" height=\"345\"><\/p>\n<h3><span style=\"color: #000000;\"><strong>\u7814\u6b21\u65b9\u6703\u7e7c\u7e8c\u8ffd\u7e31\u4e0b\u53bb\uff0c\u4e26\u5b9a\u671f\u66f4\u65b0\uff01\u540c\u6642\u975e\u5e38<span style=\"color: #ff0000;\">\u6b61\u8fce\u8f49\u767c<\/span>\u54e6\uff01\uff01<\/strong><\/span><\/h3>\n<h2><span style=\"text-decoration: underline;\"><span style=\"color: #000000; text-decoration: underline;\"><strong>\u7814\u6b21\u65b9\u7368\u5bb6\u7522\u54c1\u63a8\u85a6 \u53ca <\/strong><\/span><\/span><span style=\"text-decoration: underline;\"><strong>\u66f4\u591a\u7368\u5bb6\u6709\u8da3\u4e8b\u7269\u7cbe\u9078<\/strong><\/span><span style=\"color: #ff6600;\"><strong>\u21d3<\/strong><strong>\u21d3<\/strong><strong>\u21d3<\/strong><strong>\u21d3<\/strong><strong>\u21d3<\/strong><\/span><\/h2>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"\" src=\"https:\/\/i.imgur.com\/x332tKH.png?1\" width=\"347\" height=\"295\"><\/p>\n<p><\/p>\n<h2><span style=\"text-decoration: underline; color: #ff00ff;\"><strong><a style=\"color: #ff00ff; text-decoration: underline;\" href=\"https:\/\/fexstudio.com\/?product=%e8%88%92%e6%bd%a4%e8%86%a0%e5%9b%8a-%e9%a3%9b%e7%87%95%e8%8d%89%e7%b4%a0-%e7%82%ba%e8%91%89%e9%bb%83%e7%b4%a0-%e8%97%bb%e7%b4%85%e7%b4%a0%e7%9a%84%e4%b8%8b%e4%b8%80%e4%bb%a3-2\">\u8d85\u8d8a\u8449\u9ec3\u7d20\u8207\u85fb\u7d05\u7d20\u7684\u8b77\u773c\u795e\u5668 \u2013 MaquiBright\u98db\u71d5\u8349\u7d20<\/a><\/strong><\/span><\/h2>\n<h2><strong>\u66f4\u591a\u7368\u5bb6\u6709\u8da3\u4e8b\u7269\u7cbe\u9078\u96c6<\/strong><span style=\"color: #ff6600;\"><strong>\u21d3<\/strong><strong>\u21d3<\/strong><strong>\u21d3<\/strong><strong>\u21d3<\/strong><strong>\u21d3<\/strong><\/span><\/h2>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9ccbce0 elementor-widget elementor-widget-eael-post-timeline\" data-id=\"9ccbce0\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"eael-post-timeline.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div id=\"eael-post-timeline-9ccbce0\" class=\"eael-post-timeline timeline-layout-default eael-post-timeline-arrow-\">\n            <div class=\"eael-post-timeline eael-post-appender eael-post-appender-9ccbce0\"><article class=\"eael-timeline-post eael-timeline-column\">\n    <div class=\"eael-timeline-bullet\"><\/div>\n    <div class=\"eael-timeline-post-inner\">\n        <a class=\"eael-timeline-post-link\" href=\"https:\/\/fexstudio.com\/?p=3193\" title=\"\u70ba\u4ec0\u9ebc\u8178\u75c5\u6bd2\u6703\u9020\u6210\u5634\u5df4\u5167\u9577\u6c34\u6ce1\u5462\uff1f\">\n            <time datetime=\"2023 \u5e74 3 \u6708 25 \u65e5\">2023 \u5e74 3 \u6708 25 \u65e5<\/time>\n            <div class=\"eael-timeline-post-image  attachment-medium size-medium\" style=\"background-image: url(https:\/\/fexstudio.com\/wp-content\/uploads\/2020\/07\/licorice-root-flickr1-300x160.jpg);\"><\/div><div class=\"eael-timeline-post-excerpt\"><p>\u7518\u8349\u7d20\uff08glycyr...<\/p><\/div><div class=\"eael-timeline-post-title\">\n                    <h2 class=\"eael-timeline-post-title-text\">\u70ba\u4ec0\u9ebc\u8178\u75c5\u6bd2\u6703\u9020\u6210\u5634\u5df4\u5167\u9577\u6c34\u6ce1\u5462\uff1f<\/h2>\n                <\/div><\/a>\n    <\/div>\n<\/article><article class=\"eael-timeline-post eael-timeline-column\">\n    <div class=\"eael-timeline-bullet\"><\/div>\n    <div class=\"eael-timeline-post-inner\">\n        <a class=\"eael-timeline-post-link\" href=\"https:\/\/fexstudio.com\/?p=3211\" title=\"\u6700\u65b0\u7684\u65b0\u51a0\u80ba\u708e\u75ab\u82d7\u6e2c\u8a66\u5831\u544a\u51fa\u7210\u4e86\uff01\uff01\">\n            <time datetime=\"2023 \u5e74 3 \u6708 25 \u65e5\">2023 \u5e74 3 \u6708 25 \u65e5<\/time>\n            <div class=\"eael-timeline-post-image  attachment-medium size-medium\" style=\"background-image: url(https:\/\/fexstudio.com\/wp-content\/uploads\/2020\/07\/esomeprazole-300x167.jpg);\"><\/div><div class=\"eael-timeline-post-excerpt\"><p>\u75ab\u82d7\u51fa\u4f86\u5f8c\u624d\u6709\u51fa\u570b\u73a9...<\/p><\/div><div class=\"eael-timeline-post-title\">\n                    <h2 class=\"eael-timeline-post-title-text\">\u6700\u65b0\u7684\u65b0\u51a0\u80ba\u708e\u75ab\u82d7\u6e2c\u8a66\u5831\u544a\u51fa\u7210\u4e86\uff01\uff01<\/h2>\n                <\/div><\/a>\n    <\/div>\n<\/article><article class=\"eael-timeline-post eael-timeline-column\">\n    <div class=\"eael-timeline-bullet\"><\/div>\n    <div class=\"eael-timeline-post-inner\">\n        <a class=\"eael-timeline-post-link\" href=\"https:\/\/fexstudio.com\/?p=3398\" title=\"\u4ec0\u9ebc\uff1f\u5361\u4ecb\u82d7\u75ab\u82d7\u80fd \u201c\u6cbb\u7642\u201d \u8180\u80f1\u764c\uff1f\uff1f\uff1f\">\n            <time datetime=\"2023 \u5e74 3 \u6708 25 \u65e5\">2023 \u5e74 3 \u6708 25 \u65e5<\/time>\n            <div class=\"eael-timeline-post-image  attachment-medium size-medium\" style=\"background-image: url(https:\/\/fexstudio.com\/wp-content\/uploads\/2020\/05\/BCG-vaccine-300x179.jpg);\"><\/div><div class=\"eael-timeline-post-excerpt\"><p>\u5361\u4ecb\u82d7\u662f\u4ec0\u9ebc\uff1f\u6211\u6253\u904e...<\/p><\/div><div class=\"eael-timeline-post-title\">\n                    <h2 class=\"eael-timeline-post-title-text\">\u4ec0\u9ebc\uff1f\u5361\u4ecb\u82d7\u75ab\u82d7\u80fd \u201c\u6cbb\u7642\u201d \u8180\u80f1\u764c\uff1f\uff1f\uff1f<\/h2>\n                <\/div><\/a>\n    <\/div>\n<\/article><article class=\"eael-timeline-post eael-timeline-column\">\n    <div class=\"eael-timeline-bullet\"><\/div>\n    <div class=\"eael-timeline-post-inner\">\n        <a class=\"eael-timeline-post-link\" href=\"https:\/\/fexstudio.com\/?p=3640\" title=\"\u5fc3\u808c\u6897\u585e\u7684\u771f\u76f8\uff0c\u5fc3\u96fb\u5716\u7684\u7c21\u55ae\u5716\u793a\uff01\">\n            <time datetime=\"2021 \u5e74 7 \u6708 25 \u65e5\">2021 \u5e74 7 \u6708 25 \u65e5<\/time>\n            <div class=\"eael-timeline-post-image  attachment-medium size-medium\" style=\"background-image: url(https:\/\/fexstudio.com\/wp-content\/uploads\/2021\/07\/output_6U72fa-300x274.gif);\"><\/div><div class=\"eael-timeline-post-excerpt\"><p>\u4f60\u5e38\u5e38\u807d\u5230\u5fc3\u808c\u6897\u585e\uff0c...<\/p><\/div><div class=\"eael-timeline-post-title\">\n                    <h2 class=\"eael-timeline-post-title-text\">\u5fc3\u808c\u6897\u585e\u7684\u771f\u76f8\uff0c\u5fc3\u96fb\u5716\u7684\u7c21\u55ae\u5716\u793a\uff01<\/h2>\n                <\/div><\/a>\n    <\/div>\n<\/article><article class=\"eael-timeline-post eael-timeline-column\">\n    <div class=\"eael-timeline-bullet\"><\/div>\n    <div class=\"eael-timeline-post-inner\">\n        <a class=\"eael-timeline-post-link\" href=\"https:\/\/fexstudio.com\/?p=3564\" title=\"\u662f\u725b\u76ae\u766c\uff0c\u4e0d\u662f\u725b\u76ae\u861a\uff0c\u5b83\u4e0d\u662f\u861a\uff01\u5206\u4e94\u7a2e\uff0c\u4e00\u6b21\u770b\u660e\u767d\">\n            <time datetime=\"2021 \u5e74 7 \u6708 4 \u65e5\">2021 \u5e74 7 \u6708 4 \u65e5<\/time>\n            <div class=\"eael-timeline-post-image  attachment-medium size-medium\" style=\"background-image: url(https:\/\/fexstudio.com\/wp-content\/uploads\/2021\/07\/1625383656917-1-300x204.jpg);\"><\/div><div 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url(https:\/\/fexstudio.com\/wp-content\/uploads\/2021\/06\/\u5c01\u9762\u5716-300x167.jpg);\"><\/div><div class=\"eael-timeline-post-excerpt\"><p>\u6700\u8fd1\u77f3\u58a8\u70ef\u53c8\u6709\u4e00\u500b\u65b0...<\/p><\/div><div class=\"eael-timeline-post-title\">\n                    <h2 class=\"eael-timeline-post-title-text\">\u4ec0\u9ebc\uff1f\uff1f\u77f3\u58a8\u70ef\u651d\u5f71\u6a5f\uff5e\uff08\u4e0b\uff09<\/h2>\n                <\/div><\/a>\n    <\/div>\n<\/article><article class=\"eael-timeline-post eael-timeline-column\">\n    <div class=\"eael-timeline-bullet\"><\/div>\n    <div class=\"eael-timeline-post-inner\">\n        <a class=\"eael-timeline-post-link\" href=\"https:\/\/fexstudio.com\/?p=3455\" title=\"\u4ec0\u9ebc\uff1f\uff1f\u77f3\u58a8\u70ef\u651d\u5f71\u6a5f\uff5e\uff08\u4e0a\uff09\">\n            <time datetime=\"2021 \u5e74 6 \u6708 21 \u65e5\">2021 \u5e74 6 \u6708 21 \u65e5<\/time>\n            <div class=\"eael-timeline-post-image  attachment-medium size-medium\" 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